Application of supercritical extraction techniques to the extraction and study of proteins

Supercritical extraction is a highly efficient extraction method that uses supercritical fluids to extract active ingredients from plants. This method can extract high-quality ingredients at lower temperatures and pressures, avoiding the potential heat damage and solvent residues associated with traditional extraction methods. Supercritical extraction can be used to extract a variety of active ingredients from plants, including essential oils, terpenes, and polyphenols.

● Dahu Protein Extraction: Soybeans are an important source of plant proteins, and supercritical extraction techniques can be used to extract proteins from soybeans. By adjusting conditions such as the pressure and temperature of the supercritical fluid and adding appropriate entrainers, soybean white can be effectively separated from other components. Compared with traditional extraction methods such as alkaline capacity and acid precipitation, supercritical extraction can reduce denaturation and loss of proteins, and improve the extraction rate and the quality of strong white matter.

● Other Plant Protein Sources:In addition to soybeans, supercritical extraction can be applied to extract proteins from peanuts, walnuts, pumpkin seeds, bitter melon seeds, almonds, wheat germ, sesame seeds, moringa seeds, and other plant seeds or fruits. These plant proteins have different properties and application values, and supercritical extraction provides a new way for their efficient extraction.

● We can use supercritical extraction technology to extract proteins with specific functions from bacteria, such as enzymes with industrial applications. This method can extract proteins from bacterial fermentation solutions or mixtures after cell crushing, especially suitable for extracting heat-sensitive enzyme proteins.

● We can also use supercritical extraction technology to extract proteins from yeast cells, providing high-quality protein raw materials for hair products, feed, and other industries. By optimizing extraction parameters, we can improve the extraction efficiency and purity of yeast protein.

We can use supercritical extraction technology to extract specific protein components from animal tissues, such as blood and liver, for medical research or biological product production. However, we need to control the extraction conditions to avoid protein denaturation and loss of other biological activities.

We can use supercritical extraction technology to separate proteins from other components in complex biological samples, such as fats, polysaccharides, and nucleic acids. By adjusting the operating conditions, we can select suitable supercritical extraction conditions based on the molecular weight and polarity of proteins to achieve graded separation of different proteins.

For example, when purifying proteins from animal tissues or cell extracts, supercritical extraction can remove impurities such as fat and improve the purity of proteins. This technology is important for the study of protein structure and function and the development of specific protein products.

We can use supercritical extraction technology to extract protein-based pharmaceutical ingredients from natural medicines. Many natural medicines contain pharmacologically active protein components, which can be extracted using this technology.

For example, we can extract proteins with immunomodulatory and anti-tumor effects from medicinal plants or animal tissues, providing a new source for drug research and development.

We can also use supercritical extraction technology to prepare carrier systems for protein drugs. By encapsulating proteins in nanoparticles or microspheres using supercritical fluids, we can improve the stability and bioavailability of the drug and achieve controlled release or targeted delivery.

Compared with other techniques, supercritical extraction has the following unique advantages in protein extraction and research.

1. Selective separation: The selective extraction of target proteins can be realized by adjusting the conditions such as pressure and temperature of supercritical fluid and adding appropriate entraining agents, so as to improve the purity of proteins. The target protein can be effectively separated from other impurities, reducing the impact of impurities on subsequent research and application.

2. Green: supercritical fluid used in supercritical extraction technology (such as carbon dioxide) is a non-toxic, odorless, non-flammable gas, environmentally friendly. Moreover, the supercritical fluid can be recycled, reducing the pollution of the environment and waste of resources.

3. High extraction efficiency: Supercritical fluid has high diffusivity and solubility, which can quickly penetrate the sample, fully contact with the target protein and extract it. Therefore, supercritical extraction technology has a high extraction efficiency, can be obtained in a shorter period of time with higher protein yield.

4. Simple operation, easy to control: pressure and temperature are the key parameters in the supercritical extraction process, you can change these two parameters to control the effect of extraction and selectivity. The operation process is simple, and easy to master, according to different proteins and sample characteristics to optimize the adjustment. Supercritical extraction devices can realize automatic control, improve the productivity and stability of operation, and reduce the impact of human factors on the experimental results.

5. Save time: supercritical extraction technology is faster, generally 10 minutes of extraction, the separation of components precipitation, 2 - 4 hours or so can be completely extracted, compared with some traditional extraction methods, such as solvent extraction requires a longer extraction time and complex post-treatment steps, supercritical extraction technology can greatly shorten the extraction cycle.